fixed frozen mouse tissues

From:"Trenton R. Schoeb"

I've received an unusual request from a PI who wants to have HE sections 
made from some mouse tissues. The mice were perfused with paraformaldehyde. 
The tissues were then treated with 3 changes of 30% sucrose buffer, then 
frozen for cryosectioning. He uses this method for studies of the brain. He 
wants to look at the rest of the mouse to see if there are any other 
manifestations of the mutation he's studying. The tissues he has are 
already prepared like the brains (perfused, soaked in sucrose, and frozen). 
We could do HE stained frozens, but I wonder if we can get better fine 
detail if we paraffin embed the tissues. So, my question is, has anyone 
tried paraffin embedding and sectioning fixed frozen tissues such as these, 
and, if so, was it worth the effort? (I expect we'll have to remove the 
sucrose first.)

Trenton R. Schoeb, DVM, PhD, Diplomate ACVP
Professor, Department of Genomics and Pathobiology
University of Alabama at Birmingham
Department office: 205-934-2117
Fax: 205-975-4418

US mail:                    Fed Ex:
VH 402                      402 Volker Hall
1530 3rd Ave. S.            1670 University Blvd.
Birmingham, AL 35294-0019   Birmingham, AL 35294

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