I'm trying to do some protein localization work on intact filarial
nematodes, and we've run into a huge autofluorescence problem. The live
worms fluoresce strongly on the HeNe, Krypton and Argon lasers. We've
been fixing in 2% paraformaldehyde and that doesn't seem to be making
the fluorescence worse or better.
Any suggestions for reducing this would be great (also any reasoning
behind the suggestions would be useful).
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