From:Alanna Morris

I'm trying to do some protein localization work on intact filarial
nematodes, and we've run into a huge autofluorescence problem.  The live
worms fluoresce strongly on the HeNe, Krypton and Argon lasers.  We've
been fixing in 2% paraformaldehyde and that doesn't seem to be making
the fluorescence worse or better.

Any suggestions for reducing this would be great (also any reasoning
behind the suggestions would be useful).
Alanna Morris
Smith College

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