Hi histo folk,
I have had a question raised about the transporting of tissues in
Paraformaldehyde.
We have some samples being sent from overseas that are going to be fixed in
4% Paraformaldehyde. The concern is with them staying in PFA and becoming
'over fixed'  and the suggestion is that they should be fixed in 4% PFA for
24 hours then left in PBS till they arrive here which could be weeks or
months and we have no idea of the temperature at which they may be stored!
My feeling is to leave them in Paraformaldehyde till they get here then wash
with PBS. I have gone through the over fixing business (as discussed
recently on the histonet) but others thoughts or past experience welcomed.

Thanks
Michelle.

Michelle McAnulty-Smith
Histology Co-ordinator

NeuronZ Ltd
Tel: 09 367 7167 ext. 7060
Fax: 09 367 7186

E-mail:  michelle.mcanulty-smith@neuronz.com


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