Thawing frozen tissue for H&E
We received 3 biopsies of colon to R/O Hirschsprung's disease.
Unfortunately, all of the tissue was frozen in O.C.T. so we have nothing
for permanent sections. What is the best way to thaw the frozen tissue
before submitting it for H&E? Should we trim away the excess O.C.T. and
place it in formalin or should we "wash" it in tissue culture media
(RPMI) before placing it in formalin? Obviously, preservation of
morphology is critical here. Thanks!
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