Sections lifting off
Dear Histonetters
Here is a message Margaret is having trouble posting. She is on the
list so you may reply to the list or her directly at Mhorne@upei.ca.
Thanks.
My question is this. How can I keep breast and eyeballs sections
from lifting off the slides after heating them in the antigen retrieval
solution at 95 degrees for 20 min? I have tried silanized and poly-l-
lysine slides with sporadic success. I am now about to try gelatin
dipped . Any ideas? Thanks.
Margaret Horne
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
Canada
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