Sections lifting off

From:LINDA MARGRAF MD

Dear Histonetters
Here is a message Margaret is having trouble posting. She is on the
list so you may reply to the list or her directly at Mhorne@upei.ca.
Thanks.

My question is this.  How can I keep breast and eyeballs sections 
from lifting off the slides after heating them in the antigen retrieval

solution at 95 degrees for 20 min?  I have tried silanized and poly-l-
lysine slides with sporadic success. I am now about to try gelatin 
dipped .  Any ideas?   Thanks.
Margaret Horne 
Dept. of A&P,
Atlantic Veterinary College, U.P.E.I.
550 University Ave., Charlottetown,
P.E.I.,  C1A 4P3 
Canada





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