Re: fixed frozen mouse tissues
Dear fellow histonetters
I have limited experience on this issue of processing
frozen tissue. But I did it once and the sections came
out fine. What I did was to get the frozen tissue and
put it NBF to fix and then continued with dehydrating
clearing and embedding. I would like to hear from
those who have had experience with these kind of
tissues.
James Mubiru
UTHSCSA
--- "Trenton R. Schoeb" wrote:
> I've received an unusual request from a PI who wants
> to have HE sections
> made from some mouse tissues. The mice were perfused
> with paraformaldehyde.
> The tissues were then treated with 3 changes of 30%
> sucrose buffer, then
> frozen for cryosectioning. He uses this method for
> studies of the brain. He
> wants to look at the rest of the mouse to see if
> there are any other
> manifestations of the mutation he's studying. The
> tissues he has are
> already prepared like the brains (perfused, soaked
> in sucrose, and frozen).
> We could do HE stained frozens, but I wonder if we
> can get better fine
> detail if we paraffin embed the tissues. So, my
> question is, has anyone
> tried paraffin embedding and sectioning fixed frozen
> tissues such as these,
> and, if so, was it worth the effort? (I expect we'll
> have to remove the
> sucrose first.)
>
> --------------------------------------------------
> Trenton R. Schoeb, DVM, PhD, Diplomate ACVP
> Professor, Department of Genomics and Pathobiology
> University of Alabama at Birmingham
> trs@uab.edu
> 205-934-2288
> Department office: 205-934-2117
> Fax: 205-975-4418
>
> US mail: Fed Ex:
> VH 402 402 Volker Hall
> 1530 3rd Ave. S. 1670 University Blvd.
> Birmingham, AL 35294-0019 Birmingham, AL 35294
> --------------------------------------------------
>
>
__________________________________________________
Do You Yahoo!?
Yahoo! - Official partner of 2002 FIFA World Cup
http://fifaworldcup.yahoo.com
<< Previous Message | Next Message >>