Re: Snook's Reticulum Stain

From:Melissa Jensen

RE: Snook's Reticulum Stain
Put Sta-on in your bath water...It works most of the time.Or use coated slides..[glued or poly]
----- Original Message -----
From: Tony Henwood
To: 'JOE NOCITO' ; Fran Adams ;
Sent: Sunday, June 16, 2002 5:36 PM
Subject: RE: Snook's Reticulum Stain

Check the concentration of ammonia in your silver solution, a little too much will cause sections to lift. Also if you use permanganate/oxalic acid, try decreasing the times.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
Sent: Friday, 14 June 2002 22:32
To: Fran Adams;
Subject: Re: Snook's Reticulum Stain

how ot is yor oven?  You might want to leave the slides in the oven 10
minutes longer.  Are you microwaving the sliver?  Sometimes our GMS sections
float off, but I think it's because some of the techs try to cut corners and
shorten the time the slides are in the oven.
I also purchase my ammonia in 500 mL bottles, never had a problem, even
toward the bottom of the bottle. We usually used up one bottle evey six
months.  But then again, how fresh is fresh?  Hope I was some help.

Joe Nocito, BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, Texas
----- Original Message -----
From: "Fran Adams" <>
To: <>
Sent: Thursday, June 13, 2002 5:32 PM
Subject: Snook's Reticulum Stain

> Hi Everyone,
> Thanks for all the information you sent me on Number of Techs vs. Number
> block/slides.
> We have recently changed for doing the Wilder's Retic to the Snook's.
> For some reason we're noticing our liver biopsies washing after the silver
> solution...usually in the gold chloride.
> Has anyone else seen this particular problem when staining with the
> procedure?  We do not put the sections on "plus" slides because of the
> precipitate that forms.  We have tried leaving the freshly cut sections in
> the oven for 30 minutes.
> Also, when the Snook's calls for fresh ammonia, how fresh should it be?
> We purchase the ammonia in 500 ml bottles.  The smaller size is much more
> expensive and we do this stain almost every day.
> We would appreciate any tips you could send our way.
> Thanks,
> Fran Adams
> The Medical Center of Central GA
> Macon,GA
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