Hi Gayle;

Sorry Gayle in my haste to defend my oringinal post I didn't think to add that
information.

Journal of Bone & Joint Surgery Vol. 53A #1 1971 pg 69-82.  Rosenberg L. Chemical
Basis for the Histological use of Safranin O in the Study of Articular Cartilage

Journal of Orthopaedic Research Vol 15 1977  pg 670-674. Choji Shimizu, Richard
D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke Hirasawa and David Amiel.
Method of Histomorphometric Assessment of Glycosaminoglycans in Articular
Cartilage

The Ortho article is from our lab.  If you have any questions about it please let
me know.  We have been using this staining and analysis method for about 12
years.

Karen Bowden
Univsersity of San Diego, CA
Department of Orthopedics

Gayle Callis wrote:

> Authors and titles would help, please.
>
> Thanks
>
> At 11:38 AM 6/28/02 -0700, you wrote:
> >Yes it does. Here is a ref. to the method and a ref. to an analysis method we
> >use.
> >
> >Journal of Bone & Joint Surgery Vol. 53A #1 1971 pg 69.
> >Journal of Orthopaedic Research Vol 15 1977  pg 670-674
> >
> >Karen Bowden
> >University of San Diego, CA
> >Department of Orthopedics
> >
> >Mass Histology Service wrote:
> >
> >> I have read that the Safranin O procedure selectively stains proteoglycan
> >> (Histologic, May 2001)
> >>
> >> Jim
> >>
> >> ____________________________
> >> James E. Staruk, HT(ASCP)
> >> Mass Histology Service, Inc.
> >> www.masshistology.com
> >>
> >> -----Original Message-----
> >> From: Smith, Allen [mailto:asmith@mail.barry.edu]
> >> Sent: Friday, June 28, 2002 12:27 PM
> >> To: K. Bowden
> >> Cc: histonet@pathology.swmed.edu
> >> Subject: RE: Fwd: histonet
> >>
> >> Safranin O and toluidine blue stain the same things: all acidic substances,
> >> not just glycosaminoglycans.  Unlike alum hematoxylin, which stains
> >> nucleoproteins, safranin and toluidine blue stain nucleic acids.
> >>
> >> Allen A. Smith, Ph.D.
> >> Barry University
> >> School of Graduate Medical Sciences
> >>     Podiatric Medicine and Surgery
> >> Miami Shores, Florida  33161-6695
> >>
> >> -----Original Message-----
> >> From: K. Bowden [mailto:kbowden@ucsd.edu]
> >> Sent: Thursday, June 27, 2002 4:21 PM
> >> To: John Baker; histonet@pathology.swmed.edu
> >> Subject: Re: Fwd: histonet
> >>
> >> Safranin O stains the glyocosaminoglycans which is what the tol blue (pH
> >> 4.1) stains.  If you don't have glyocosaminoglycans present then
> >> Safranin O with not stain.  I find that a good H&E shows the line of
> >> demarcation best.
> >>
> >> Karen Bowden
> >> UCSD
> >> Orthopedic Department
> >>
> >> John Baker wrote:
> >>
> >> > Histonetters:
> >> >    I have a question regarding a staining technique:
> >> > >Does a saf O/fast green counterstain add anything to a tol blue
> >> > >stain?  I am evaluating dimensions of a growth plate and want a
> >> > >readily identifiable demarcation between cartilage and bone as well
> >> > >as between the various layers of the growth plate. Distinct nuclei
> >> > >would be an added bonus.  Thank you very muc in advance or any
> >> > >responses.
> >> > >
> >> > >Dr. Ryan Frank    via John Baker
> >> >
> >> > --
> >> > John A. Baker
> >> > The University of Michigan
> >> > Orthopaedic Research Laboratories
> >> > Histology Unit
> >> > 400 North Ingalls, G160
> >> > Ann Arbor, MI 48109-0486
> >> > Main lab office phone:734-763-9674
> >> > Histology office:734-936-1635
> >
> >
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367 (voice mail)
> 406 994-4303 (FAX)
>
> email: UVSGC@montana.edu