Hello Marilyn,
I use Dako's ready to use Prot. K. cat# S3020, that's a 15ml bottle and they also have a larger bottle. It is fantastic.  When I use it, it's at room temperature for and usually for 5 minutes.  On some protocols I need it longer, 10 minutes RT
Dana Settembre
University Hospital- UMDNJ
Newark, NJ
USA

>>>  06/20/02 02:02PM >>>
Hi Histonetters,
I need some references for the use of proteolytic digestion methods, in
particular using Proteinase K, used in Immunohistochemical staining
methods. Other reagents such as trypsin and protease would also be greatly
appreciated.
Thankyou in advance.

Marilyn Johnson
Agri-Food Surveillance Systems
Alberta Agriculture
Edmonton, Alberta, Canada