Re: Mounting media for toluidine blue stained slides
Silvia, our epoxy sections, stained with toluidine blue + basic fuchsin are
coversliped with the same embedding media, spurr epoxy.
Reference. STAT HIGH RESOLUTION LIGHT MICROSCOPY (HRLM) - TRANSMISSION
ELECTRON MICROSCOPY (TEM) TECHNIQUES FOR TRANSPLANT RENAL BIOPSIES: The
Journal of Histotechnology / Vol. 21, No. 3/ September 1998 page 214 under
Hope this helps, Teresa
>I'm cutting some semi-thin sections (1 micron) from Epon-embedded tissues.
>I'd like to know what people use for mounting slides of toluidine blue
>I noticed that there was a bleaching of sections after serial passage in
>alcol 70%, 95%, 100%, xylene to DPX. Is better to use an aqueous medium (for
>example Glycerol gelly )? Are there significant differences in mounting
>among epoxidic resins (for example Epon 812 versus Spurr's )?
>Thanks in advance
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