Re: CD31 in FFPE mouse tissues


I have use Research Diagnostics anti Rat CD31 on zinc formalin fixed PE rat tissue
with success.  I didn't reply before because you were asking for experience on
mouse tissue.  I did a poster on Endothelial Cells staining at the NSH S/C last
year in Charlotte.  You can find a description of the poster on the IHCRG web site
feel free to join our group online while visiting our web site.
Patsy Ruegg wrote:

> Teri,
> I just completed a project usind CD31 on FFPE mouse tissue with good results.
> The supplier of the antibody is Research Diagnostics (973-584-7093;
>  I used rat anti-mouse CD31, monoclonal; Catalog#
> RDI-mCD31abrt.  They also suggested zinc fixation, but it worked on formalin
> fixation.  I used DAKO's Prot K to open up the sights.
> Bob Meyer
> Pathology Core Facility
> Northwestern University
> >
> > I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin embedded
> mouse tissue.  I understand some companies offer antibodies that don't work
> well in formalin fixed material, but instead suggest using zinc fixed tissue
> (not zinc formalin fixed).  Unfortunately, I don't happen to have any zinc
> fixed tissue at the moment.
> >
> > Thanks in advance!
> >
> > Teri Johnson
> > Managing Director Histology Core Facility
> > Stowers Institute for Medical Research
> > 1000 E. 50th St.
> > Kansas City, Missouri  64110
> >
> >
> >

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