Date sent:      	Mon, 17 Jun 2002 09:04:25 -0500 (CDT)
From:           	r-meyer2@northwestern.edu
Subject:        	CD31-- Human tumors in mice
To:             	histonet@pathology.swmed.edu


Does anyone know of a CD31 antibody that will cross react with human tumors in 
mice??  I tried DAKO's CD31 which I thought would work because these are human 
tumors.  The human tonsil control was great but the human tumors in mice were 
all negative.  Any help is much appreciated. 

Bob, if you are looking at human xenografts in mouse tissue and 
studying the vascular response then you will need an antibody to 
the host tissue, i.e. mouse. The one that I found worked well in this 
situation was CD 31, clone MEC 13.3 produced in rat, from 
PharMingen.
Terry.

Terry Hacker,
Head of Histopathology and Electron Microscopy
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 824521
Fax 01235 834776
e-mail t.hacker@har.mrc.ac.uk