Hi! I did the  HAVA procedure today.I wasnt pleased with the back ground
staining.Basically there wasnt any.I rechecked the procedure..I couldnt find
an error.So I looked in Bancroft.The proceedure you were kind enough to send
me is in there.Anyway..I saw in the print out of the stain..10%formalin..I
used 10% nbf.In the book it said 10% formalin in water.Im guessing I was
suppose to make a 10% solution of 37% formaldehyde???..The stain itself
worked great! Demonstrated plaque!                                 -----
Original Message -----
From: "McMeekin, Bill" 
To: "'Melissa Jensen'" 
Cc: "'Histonet (list)'" 
Sent: Monday, June 17, 2002 4:16 AM
Subject: RE: Alzheimers stain


> Hi Melissa
>
> HAGA is the name I have seen attached to the modified methenamine silver
> method for cerebral amyloid that I mentioned.  I have assumed it was a
> contraction of 'Hexamine-(H)-Silver-(AG)-(A)'.  Let me know if you need to
> see a method.  As regards cost, it uses a methenamine-silver solution that
> is similar to that in a Gomori-Grocott but does not require the initial
> chromic acid oxidation step  It requires reduction with formaldehyde after
> impregnation but no gold toning.
>
> Bill McMeekin
> Senior Chief Biomedical Scientist
> Neuropathology
> Newcastle General Hospital
> UK
>
>
> -----Original Message-----
> From: Melissa Jensen [mailto:melzy@indytel.com]
> Sent: 15 June 2002 23:08
> To: histonet@pathology.swmed.edu; McMeekin, Bill
> Subject: Re: Alzheimers stain
>
>
> Bill
>    What is HAGA?
> ----- Original Message -----
> From: "McMeekin, Bill" 
> To: "'Melissa Jensen'" 
> Sent: Friday, June 14, 2002 5:45 AM
> Subject: RE: Alzheimers stain
>
>
> > Melissa
> >
> > You can stain for plaques and tangles separately.  The modified
> methenamine
> > silver method (HAGA) is a simple and reliable (for a silver) technique
to
> > stain senile plaques and diffuse amyloid in AD brain (and if you do
> Gomori's
> > methenamine silvers for fungi etc. you will have the solutions already).
> We
> > use the Gallyas method as a silver impregnation method for
neurofibrillary
> > tangles in AD brain and this gives excellent contrast with no background
> > staining (but is a bit more complex and uses slightly more exotic
> > solutions).  I would use IHC for beta-Amyloid and PHF-Tau (AT8) by
> > preference.
> >
> > Bill McMeekin
> > Senior Chief Biomedical Scientist
> > Neuropathology
> > Newcastle General Hospital
> > UK
> > -----Original Message-----
> > From: Melissa Jensen [mailto:melzy@indytel.com]
> > Sent: 13 June 2002 23:49
> > To: histonet@pathology.swmed.edu; Joan Yonchek
> > Subject: Re: Alzeheimers stain
> >
> >
> >
> > Thanks much Joan! I am wondering now if there is a different silver
method
> > to use.The reagents for Bielsowskys are numerous.Not sure I want to
spend
> > that kind of money for a stain we may only use occasionally.Any
> > recommendations you have would be appreciated!
> >
> > ----- Original Message -----
> > From: Joan Yonchek 
> > To: 'Melissa Jensen' 
> > Sent: Thursday, June 13, 2002 4:38 PM
> > Subject: RE: Alzeheimers stain
> >
> > Melissa,
> >
> > Just sent the fax.  If you try the procedure let me know how it turns
out.
> >
> > Good luck,
> >
> > Joan
> > Regeneration Technologies, Inc
> > Alachua, FL  32615
> >
> > -----Original Message-----
> > From: Melissa Jensen [mailto:melzy@indytel.com]
> > Sent: Wednesday, June 12, 2002 6:33 PM
> > To: Joan Yonchek
> > Subject: Re: Alzeheimers stain
> >
> >
> > That would be great! Fax is 319-366-6976
> >
> > ----- Original Message -----
> > From: Joan   Yonchek
> > To: 'Melissa Jensen' 
> > Sent: Tuesday, June 11, 2002 7:59 AM
> > Subject: RE: Alzeheimers stain
> >
> > Melissa,
> >
> > Microwave Cookbook for Microscopists
> > Art and Science of Visualization
> > By  Kok and Boon
> >
> > Chapter 16, page 250
> > Procedure 16.15  Microwave-Stimulated King Silver Staining for
> > paraffin-embedded tissues.
> > Results:  Neurofibullary tangles and senile plaques are black.
> > The procedure states:  The core of the plaque is difficult to stain.
The
> > Alzheimer's fibrils will not always be colored.  Lloyd et al. published
in
> > 1985 a microwave method. We changed their method slightly.
> >
> > I have never tried this procedure but would be happy to fax you a copy.
> >
> > Joan
> > Regeneration Technologies, Inc
> > Alachua, FL  32615
> >
> >
> >
> >
> >
> > At 6:21 PM -0500 9/6/02, Melissa Jensen wrote:
> >
> > Looking for a microwave stain to demonstrate plaque in the brain.....Can
> ya
> > help me out?
> >
> >
>