RE: help w/ cornea staining

From:"Monson, Frederick C."

Hi Tamara,

In times past when having trouble with eosin 'fasatness', I have resorted to
skipping the 95% rinse and going to 100% directly.  When using alcoholic
Eosin, this works like a charm to 'keep' the eosin in the tissue where it is
wanted.  I would hope it might do the same for aqueous eosin.  Right from
the stain to 100%!

Hope this is of help,

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone:  610-738-0437
FAX:  610-738-0437
Visitors URL:

> ----------
> From: 	Tamara Howard
> Sent: 	Tuesday, June 18, 2002 3:02 PM
> To: 	HistoNet
> Subject: 	help w/ cornea staining
> I need help!! I'm trying to stain cornea sections (formaldehyde-fixed,
> embedded in paraffin) for several things, but am having the most trouble
> with the "plain" staining - my H&Es are garbage. I've tried several
> formulations for both stains and several different staining protocols, but
> the collagen isn't holding the eosin very well when I go back to dehydrate
> before mounting (the hematoxylin is fine). Any tricks to mordant the
> eosin? Anyone have another favorite cornea stain (other than H&E)? We just
> need sort of a survey slide for orientation, but the MD wants to see
> pretty collagen. The stains themselves are fine (no trouble with other
> samples) and other blocks that were processed at the same time as the
> corneas are fine.
> Any help will be greatly appreciated and will drastically cut my aspirin
> consumption!
> Tamara
> |--------------------------------------------------|
>  Tamara Howard
>  Department of Cell Biology and Physiology
>  University of New Mexico - Health Sciences Center
>  Albuquerque, NM 87131
> |--------------------------------------------------|

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