RE: Whole mouse snap freezing for frozen sections

From:Abizar Lakdawalla

RE: Whole mouse snap freezing for frozen sections

Hi Gayle, how do you cryosection (what thickness, what type of ...tome?).
Thanks in advance,
Abizar

> -----Original Message-----
> From: Gayle Callis [mailto:uvsgc@montana.edu]
> Sent: Tuesday, June 25, 2002 1:38 PM
> To: histonet@pathology.swmed.edu
> Subject: Whole mouse snap freezing for frozen sections
>
>
> I was asked how people snap freeze a euthanized whole mouse for serial
> frozen sections.  I've snap frozen whole mouse heads, tibias,
> femurs using
> dry ice cooled hexanes after coating the samples with either
> diluted OCT or
> 4% PVA, 30,000 - 70,000 water soluble from Sigma. Is this
> snap freezing
> method going to be too slow for a whole mouse. Any help would be
> appreciated.  Off to PubMed.
>
> Searching the Archives was not turning up the answer
>
>
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367 (voice mail)
> 406 994-4303 (FAX)
>
> email: UVSGC@montana.edu
>
>


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