RE: Tunnel Stain

From:ajennings@unmc.edu


guess this post was missed by a couple people so here it is again, I still
need the antibodies and still like the kit

 I need antibodies to Somatostatin and GM-CSF (granulocyte macrophage
 colony stimulating factor), which work in frozen mouse tissue. Any
 suggestions will be appreciated.
Also I would like to thank everyone who gave me input into my Tunnel assay
question, especially whoever it was that recommended the Roche (1 684 795)
In situ death detection kit to me.For the numerous collegues who asked for
an update if I got it to work.........Not only was it easy to use, I am now
confident enough in the repeated experiments to recommend it to others
doing apoptotic studies in paraffin embedded mouse tissues, (we looked at
bone marrow in whole leg samples). It needed a little tweaking but the
obvious difference between the experimental and control groups is a no
brainer to quantitate. good luck
Anita


                                                                                                                            
                    "Dunn-Jena,                                                                                             
                    Patsy Ann"           To:     Linresearch@aol.com, histonet@pathology.swmed.edu                          
                                   Subject:     RE: Tunnel Stain                                                      
                                                                                                                            
                    06/20/2002                                                                                              
                    06:46 AM                                                                                                
                                                                                                                            
                                                                                                                            




I could also use this information as a student is having problems with it,
but has not consulted me prior to this regarding his problems.  I just
recently found out.

Patsy Dunn-Jena
Indiana University School of Dentistry
Mineralized Tissue and Histology Research Laboratory
padunnje@iupui.edu










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