RE: Thawing frozen tissue for H&E

From:Tony Henwood

RE: Thawing frozen tissue for H&E

What we do with Hirschsprung's frozen biopsies is place the frozen block directly into 10% NBF and process as usual. If there is not freezing problem, then the morphology should be OK

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Richard Cartun []
Sent: Saturday, 22 June 2002 4:33
Subject: Thawing frozen tissue for H&E

We received 3 biopsies of colon to R/O Hirschsprung's disease.
Unfortunately, all of the tissue was frozen in O.C.T. so we have nothing
for permanent sections.  What is the best way to thaw the frozen tissue
before submitting it for H&E?  Should we trim away the excess O.C.T. and
place it in formalin or should we "wash" it in tissue culture media
(RPMI) before placing it in formalin?  Obviously, preservation of
morphology is critical here.  Thanks!

R. Cartun

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