RE: Sections lifting off

From:PMarcum

I don't think there is much to add to Aidan's very precise comments of Poly
L Lysine slides and sticking.  Silane charged should be treated the same way
for the hold.  Pam Marcum

> -----Original Message-----
> From: Aidan Schurr [mailto:Aidan.Schurr@hvh.co.nz]
> Sent: Thursday, June 27, 2002 7:15 PM
> To: histonet@pathology.swmed.edu; Mhorne@upei.ca
> Subject: Re: Sections lifting off
>
>
> Poly-l-lysine slides should in theory stick down just about any
> kind of well processed tissue... so if tissues aren't sticking
> well, the first thing to look at is "are they properly
> processed?".  If so, lets look a little further afield.  How are
> you draining and drying the slides?  Any coated slides need to be
> *completely* drained before going near heat, otherwise small
> amounts of water get stuck under the section.  For ours we drain
> the slides vertically at room temperature for a minimum of 1hr,
> then into 37C incubator overnight.  Cook on at 60C for 20
> minutes before staining the next morning (with the positive
> controls to ensure uniformity) to make sure they are stuck.
> Still no luck?  Look at the water in your floating out bath...
> Are you using distilled?  Tap water just won't do in my
> experience.  Are you using any additives in the water bath?
> These are an absolute no-no when it come to coated slides.
> Eliminating the additive from our waterbaths about 12 months ago
> cured our lifting problems overnight.
>
> Hope all this is of some help.  I know first hand just how
> frustrating it can be!
>
> Cheers,
> Aidan
>
>
> __
>
> aidan schurr  b.m.l.sc
> section head, histology
> hutt valley district health board
> lower hutt
> new zealand
>
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173 (direct)
> ++64 4 570 9214 (fax)
>
> >>> LINDA MARGRAF MD  28/06/2002 >>>
> Dear Histonetters
> Here is a message Margaret is having trouble posting. She is on the
> list so you may reply to the list or her directly at Mhorne@upei.ca.
> Thanks.
>
> My question is this.  How can I keep breast and eyeballs sections
> from lifting off the slides after heating them in the antigen retrieval
>
> solution at 95 degrees for 20 min?  I have tried silanized and poly-l-
> lysine slides with sporadic success. I am now about to try gelatin
> dipped .  Any ideas?   Thanks.
> Margaret Horne
> Dept. of A&P,
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave., Charlottetown,
> P.E.I.,  C1A 4P3
> Canada
>
>
>
>
>






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