Hi Carol,

I have always used 70% ethanol that is 10-20% glycerol to help retard
evaporation.  [Of course, you muscle people might not like all that glycerol
around, so you might substitute some isopropanol and/or some tertiary
butanol.]  Then I put on a screw cap over a layer of Parafilm@, trim off the
excess and invert the cap in my paraffin bath to give a good seal.  I have
not been as happy as a lark about the paraffin over the long haul, so, in
your place I might use some melted beeswax from a lit candle while I rotated
the jar underneath to get the melt in the cap-to-jar space.  Using a hot air
blower with a small orifice, you might get an even better seal - if you
don't want to open the thing for the next 20 years or so.

Hope this helps, even though its Friday and Germany won [BUT NOT by a rout.
Just by their usual style and quality of play.  USA in when?  2010 or 2014?
We may be much better, but can anyone be faster than those Brazilians?  And
can I live/hold on that long?]

Have a good weekend all,

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging
Schmucker II Science Center
West Chester University
South Church Street and Rosedale
West Chester, Pennsylvania, USA, 19383
Phone:  610-738-0437
FAX:  610-738-0437
fmonson@wcupa.edu
CASI URL:  http://darwin.wcupa.edu/casi/
WCUPA URL:  http://www.wcupa.edu/
Visitors URL:  http://www.wcupa.edu/_visitors/


> ----------
> From: 	Carol Thouron
> Sent: 	Friday, June 21, 2002 10:16 AM
> To: 	histonet
> Subject: 	Preservative
> 
> I have been asked to recommend a non formalin/phenol fluid for the
> preservation of  a giraffe heart.  It has been fixed in formalin but
> cannot be stored in any type of fixative that I have suggested (safety
> regulations of the institute).  Any ideas or suggestions are appreciated.
>  
> C. Thouron
> LSU Health Sciences Center
>  
>