RE: Myogenin control


We use Myogenin on our Ventana stainer also - a Dako product - and have not experienced loss of antigenicity in our room temp stored control slides - yet.

Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454

>>> "Drew Sally A."  06/17/02 07:27AM >>>
One thing I know about myogenin is that the controls must be either cut
fresh or kept frozen-it's one of those that "loses it" if kept at room temp.
Our antibody is from BioCare Medical, our controls are all rhabdos...and we
run our slides on the Ventana stainers.  If you'd like further info, you're
more than welcome to contact me.

Sally Ann Drew-MT(ASCP)
IHC/ISH Clin./Res. Laboratory
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472

-----Original Message-----
From: Martha Ward [] 
Sent: Monday, June 17, 2002 7:35 AM
Subject: Myogenin control

Martha Ward writes:

I am in need of a good positive control for myogenin.  I have tried
several blocks and am not getting very good results.  We used to get
good staining and now...  nothing.  Not sure what is going on but would
appreciate any input, including what antibody, dilution, pre-treatment,
etc.  We have been using one from Santa Cruz Biotechnology.  Thanks in
advance for your help!

Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157

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