RE: Mounting media for toluidine blue stained slides

From:"Monson, Frederick C."

Morning Silvia,

As toluidine Blue is metachromatic, and ethanols are noted for their
capacity to dissociate the dye-mers that are responsible for the
metachromatic shifts of these substances, I long ago, without a reference,
developed the practice of staining, redrying the specimen and mounting
directly in xylene/dammar (low viscosity) with a 22x22mm #1 coverslip.  The
specimens that I did in the 80's still look pretty good.  

Once you have stained the plastic-embedded sections, for a time that
provides good staining with only a water rinse, you are in position to
simply dry the sections and mount.  Also, you are probably de-staining, not
bleaching, by using the alcohols.  

My method is an old method and does NOT account for the refractively
friendly plastic thermoplastic mountants that are currently on the market
(and I have NOT used, yet!). [a friend of Dr.
Garber's, only!]

Hope this helps.

> ----------
> From: 	Pasquetto, Silvia
> Sent: 	Tuesday, June 25, 2002 8:42 AM
> To: 	Histonet chat (E-mail)
> Subject: 	Mounting media for toluidine blue stained slides
> Dear 'netters,
> I'm cutting some semi-thin sections (1 micron) from Epon-embedded tissues.
> I'd like to know what people use for mounting slides of toluidine blue
> stained tissues. 
> I noticed that there was a bleaching of sections after serial passage in
> alcol 70%, 95%, 100%, xylene to DPX. Is better to use an aqueous medium
> (for
> example Glycerol gelly )? Are there significant differences in mounting
> among epoxidic resins (for example Epon 812 versus Spurr's )?
> Thanks in advance
> Regards,
> Silvia
> GSK Company

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