RE: CD31 in FFPE mouse tissues

From:Luis Chiriboga

Do you recall the clone ??

-----Original Message-----
From: r-meyer2@northwestern.edu [mailto:r-meyer2@northwestern.edu]
Sent: Friday, June 28, 2002 8:40 AM
To: histonet@pathology.swmed.edu
Subject: Re: CD31 in FFPE mouse tissues



Teri,

I just completed a project usind CD31 on FFPE mouse tissue with good
results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com).  I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt.  They also suggested zinc fixation, but it worked on formalin
fixation.  I used DAKO's Prot K to open up the sights.

Bob Meyer
Pathology Core Facility
Northwestern University

>
> I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin
embedded
mouse tissue.  I understand some companies offer antibodies that don't work
well in formalin fixed material, but instead suggest using zinc fixed tissue
(not zinc formalin fixed).  Unfortunately, I don't happen to have any zinc
fixed tissue at the moment.
>
> Thanks in advance!
>
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110
> tjj@stowers-institute.org
>
>










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