RE: Bone Marrow Iron Stain.


EQA work, in the UK,  has shown wide variation in the quality of Perls
staining.  In my own department where we use a mix of 10% HCL and 10% Pot.
Ferrocyanide we found three rules:-

Make up the ferrocyanide solution just before use.

Incubate in ferrocyanide for 10 minutes before incubating in the mix.

While the HCL doesn't need to be fresh it should't be ancient.

Never used "Accustain" so cannot comment.

Andy Shand 

-----Original Message-----
From: Baker, Christine [mailto:CHRISTINE.BAKER@TENETHEALTH.COM]
Sent: 27 June 2002 17:10
Subject: Bone Marrow Iron Stain.

I am having a problem with my bone marrow iron stain, I hope someone can
help. My pathologist's are complaining that a disproportionate number ore
showing negative staining. The clinical history does not support a negative
stain either. The onocologists also are having a hard time believing that
these are negative.
 I am using Sigma's Accustain, which is essentially a Prussian Blue
reaction.  I have tried a lot of other iron stains including making my own.
I use an in house bone marrow iron control which stains ok. We collect our
bone marrows in 10% buffered formalin. I have used B5 etc nad stopped
thinking that might be part of the problem. We also stain a smear with the
clot section of the so I doubt fixation is a problem.  I have used a tissue
sectiojn for control. it also was ok.
  I am hoping someone has an answer.
Christine Baker
Seven Rivers Community Hosp
Crystal River Florida

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