Silvia,
            Dry the stained sections and mount using immersion oil. If I
decided to keep the mounted sections then I'd ring with ringing agent or
nail varnish. I also use Entellan but immersion is my first favourite.
Ian.


Dr. Ian Montgomery,
Histotechnology,
Academic Support Unit,
Graham Kerr Building,
Institute of Biomedical and Life Sciences,
University of Glasgow,
Glasgow,
Scotland,
G12 8QQ.
Tel: 0141 339 8855.
Lab: 6644.
Office: 4652.
Fax: 0141 330 5971
e-mail: ian.montgomery@bio.gla.ac.uk


----- Original Message -----
From: "Pasquetto, Silvia" 
To: "Histonet chat (E-mail)" 
Sent: Tuesday, June 25, 2002 1:42 PM
Subject: Mounting media for toluidine blue stained slides


Dear 'netters,
I'm cutting some semi-thin sections (1 micron) from Epon-embedded tissues.
I'd like to know what people use for mounting slides of toluidine blue
stained tissues.

I noticed that there was a bleaching of sections after serial passage in
alcol 70%, 95%, 100%, xylene to DPX. Is better to use an aqueous medium (for
example Glycerol gelly )? Are there significant differences in mounting
among epoxidic resins (for example Epon 812 versus Spurr's )?

Thanks in advance

Regards,
Silvia
GSK Company