FW: Mounting media for toluidine blue stained slides
Thanks to many people who answered to my post. Now, I have a more complete
view about this topic.
Thanks again.
Silvia
> -----Original Message-----
> From: Pasquetto, Silvia
> Sent: marted́ 25 giugno 2002 14:42
> To: Histonet chat (E-mail)
> Subject: Mounting media for toluidine blue stained slides
>
> Dear 'netters,
> I'm cutting some semi-thin sections (1 micron) from Epon-embedded tissues.
>
> I'd like to know what people use for mounting slides of toluidine blue
> stained tissues.
>
> I noticed that there was a bleaching of sections after serial passage in
> alcol 70%, 95%, 100%, xylene to DPX. Is better to use an aqueous medium
> (for example Glycerol gelly )? Are there significant differences in
> mounting among epoxidic resins (for example Epon 812 versus Spurr's )?
>
> Thanks in advance
>
> Regards,
> Silvia
> GSK Company
>
>
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