We have to stain for both gram positive and negative micro-organisms in the same paraffin section.  However, we usually do conventional gram and therefore not too familiar with the Twort's or the Brown Hopps method.  Which one is more reproducible and easier to master?  How do these methods compare in yours hands?   As for the piciric acid-acetone employed in Brown Hopps, do you think this counterstain is going to further decolorize the gram positive organisms and nuclei?  As I have never seen a section stained with Brown Hopps before, can anyone tell me what the expected results are?  I have tried both methods.  With the Brown Hopps, nuclei stain a brownish (not bright at all) red and in the same colour and intensity as gram negative organisms.  Is that OK?  It would be very nice if anyone can his or her experience with me.  Thanks in advance.
Helen Lam
Tuen Mun Hospital,
Hong Kong

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