Bone Marrow Iron Stain.

From:"Baker, Christine"

I am having a problem with my bone marrow iron stain, I hope someone can help. My pathologist's are complaining that a disproportionate number ore showing negative staining. The clinical history does not support a negative stain either. The onocologists also are having a hard time believing that these are negative.
 I am using Sigma's Accustain, which is essentially a Prussian Blue reaction.  I have tried a lot of other iron stains including making my own.
I use an in house bone marrow iron control which stains ok. We collect our bone marrows in 10% buffered formalin. I have used B5 etc nad stopped thinking that might be part of the problem. We also stain a smear with the clot section of the so I doubt fixation is a problem.  I have used a tissue sectiojn for control. it also was ok.
  I am hoping someone has an answer.
Christine Baker
Seven Rivers Community Hosp
Crystal River Florida

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