toxicity in brain

I hope you folks will forgive a naive question from a psychologist who does
some histology & is trying to help another psychologist who knows even less
about histology:  she wants to use H&E to assess toxicity in rat brain, but
in fresh-frozen brain (not yet sectioned).  I've provided her with a
protocol for H&E, but can the slide-mounted sx's be fixed adequately at that
stage for H&E to work?  Or might there be another stain or assay that would
work better for this purpose?  Thanks for any advice!   Susan

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