re:antibody diluent


Certainly, the only thing to watch for is that your primary diluent may
contain sodium azide as a preservative; if it does, don't use it for any
secondaries or tertiaries which have peroxidase conjugated to them - azide
inhibits peroxidase.
Lawrence Brett
Immunocytochemistry Lab.
Pathology Dept.
Western General Hospital
Crewe Road South
Edinburgh  EH4 2XU
Tel: 0131 537 2148

> Hi All
> I am sick of spending a zillion dollars on IHC kits, and I have decided to
dilute my own.  Does anyone know....can I use primary antibody diluent for
my secondaries?

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