cytological preparations

From:Pat Paulusse

Hello All,
Are there any established protocols or practices for the preparation of non-gyn cytology specimens.  What I am looking for is not the actual preparation procedure but the guidlines for each particular specimen. For example, for sputums - are 2 slides enough, or four slides, or just a block preparation?  Are two, four, or six slides acceptable for a fine needle aspiration, etc.
Also, when staining these specimens (I use a Leica Autostainer) can the non-gyn specimens be batched or should each case be stained separately to prevent carry-over. 
I am trying to establish protocols for the Cytology lab which now prepares specimens on a case by case basis.  If there are no established protocols, what does everyone else do?  My main goal is to standardize procedures and to make the deparment more efficient.  Any suggestions would be appreciated.  
Patrick Paulusse
Histology Supervisor
Pembroke General Hospital
Ontario, Canada
(613)732-3675 ext 6716

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