Re: toxicity in brain

From:Nancy E Weber

Hi Susan,

Here's a procedure for frozens I've used that works pretty well.   Hope this
helps,  Nancy

After cutting fix in a sol'n of 3 parts methanol to 1 part 37% formaldehyde
with 6 drops of glacial acetic acid added just before using for 3 -4 mins.
1. Rinse the slides in running water.
2, Stain with Mayer's hematoxylin for 3.5 min.
3. Rinse in running water for 2 min or until blue.
4. Rinse in 80% ethanol.
5. Counterstain in eosin for 15 sec.
6. Differentiate through 2 changes of 95% ethanol, dehydrate with 100%
ethanol and clear.

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
To: "'Histology Network'" 
Sent: Thursday, June 06, 2002 3:39 PM
Subject: toxicity in brain

> I hope you folks will forgive a naive question from a psychologist who
> some histology & is trying to help another psychologist who knows even
> about histology:  she wants to use H&E to assess toxicity in rat brain,
> in fresh-frozen brain (not yet sectioned).  I've provided her with a
> protocol for H&E, but can the slide-mounted sx's be fixed adequately at
> stage for H&E to work?  Or might there be another stain or assay that
> work better for this purpose?  Thanks for any advice!   Susan

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