Re: cytological preparations
Negative non-gyn cases could be
batched to be stained. At the hospital that I last worked at we screened all
non-gyn cases for malignancy. While preparing the smears a drop of the
sediment was put on a slide with a drop of toluidine blue and a coverslip
lowered on (a wet prep). Either a cytotech or a pathologist would quickly
screen these slides for malignancy before staining the prepared smears.
Any cases that were malignant or suspicious for malignancy were stained
separately to prevent cross contamination. Solutions used for the malignant
cases were filtered after use or disposed of. A good set up for the
malignant cases are the plastic 5-slide mailers. They hold about 20 mL of
stain and the filtering is easier or disposal much less that using the
traditional coplin jars or staining containers.
We prepared 4 smears and a cell block (if possible)
on all non-gyn cases. Two smears were stained with the Diff-Quik procedure
and two were stained with the PAP stain. An H&E was done on the cell
FNA cases depended on the amount of material
available. We usually received slides from a doctor's office or our
Pathologist performed the FNA and he determined the number of
Hope this helps.
Mt San Antonio College
Are there any established protocols or practices for the
preparation of non-gyn cytology specimens. What I am looking for
is not the actual preparation procedure but the guidlines for each
particular specimen. For example, for sputums - are 2 slides enough, or
four slides, or just a block preparation? Are two, four, or six slides
acceptable for a fine needle aspiration, etc.
Also, when staining these specimens (I use a Leica
Autostainer) can the non-gyn specimens be batched or should each case
be stained separately to prevent carry-over.
I am trying to establish protocols for the Cytology lab
which now prepares specimens on a case by case basis. If there are no
established protocols, what does everyone else do? My main
goal is to standardize procedures and to make the deparment more
efficient. Any suggestions would be
Pembroke General Hospital
(613)732-3675 ext 6716
<< Previous Message | Next Message >>