Re: antibody diluent
I do. I use normal serum from the species in which the secondary is made.
I also use that as my blocker (including 2-5% BSA) and the primary
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
On Thu, 6 Jun 2002, Linke_Noelle wrote:
> Hi All
> I am sick of spending a zillion dollars on IHC kits, and I have decided to dilute my own. Does anyone know....can I use primary antibody diluent for my secondaries?
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