Dear Linke_Noelle

I do.  I use normal serum from the species in which the secondary is made.
I also use that as my blocker (including 2-5% BSA) and the primary
antibody diluent.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Thu, 6 Jun 2002, Linke_Noelle wrote:

> Hi All
>
> I am sick of spending a zillion dollars on IHC kits, and I have decided to dilute my own.  Does anyone know....can I use primary antibody diluent for my secondaries?
>
>