Fran,
how ot is yor oven?  You might want to leave the slides in the oven 10
minutes longer.  Are you microwaving the sliver?  Sometimes our GMS sections
float off, but I think it's because some of the techs try to cut corners and
shorten the time the slides are in the oven.
I also purchase my ammonia in 500 mL bottles, never had a problem, even
toward the bottom of the bottle. We usually used up one bottle evey six
months.  But then again, how fresh is fresh?  Hope I was some help.

Joe Nocito, BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, Texas
----- Original Message -----
From: "Fran Adams" 
To: 
Sent: Thursday, June 13, 2002 5:32 PM
Subject: Snook's Reticulum Stain


> Hi Everyone,
>
> Thanks for all the information you sent me on Number of Techs vs. Number
of
> block/slides.
>
> We have recently changed for doing the Wilder's Retic to the Snook's.
>
> For some reason we're noticing our liver biopsies washing after the silver
> solution...usually in the gold chloride.
>
> Has anyone else seen this particular problem when staining with the
Snook's
> procedure?  We do not put the sections on "plus" slides because of the
> precipitate that forms.  We have tried leaving the freshly cut sections in
> the oven for 30 minutes.
>
> Also, when the Snook's calls for fresh ammonia, how fresh should it be?
> We purchase the ammonia in 500 ml bottles.  The smaller size is much more
> expensive and we do this stain almost every day.
>
> We would appreciate any tips you could send our way.
>
> Thanks,
> Fran Adams
> The Medical Center of Central GA
> Macon,GA
>
>
>
>
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