Re: Pretreatment for in situ hybridization
----- Original Message -----
From: "C Sawrenko"
To: "HistoNet Server"
Sent: Thursday, June 06, 2002 8:35 PM
Subject: Pretreatment for in situ hybridization
> Hello Histonetters,
> Does anyone out there have any experience using heat (by steam, microwave
> pressure cooker) instead of enzyme (Proteinase K) for pretreatment prior
> in situ hybridization? We are attempting ISH for kappa, lambda and EBER.
> Also has anyone had any success on B5 fixed tissue for the above?
> Thanks in advance for your help!
> Christina Sawrenko
> BC Cancer Agency
> Vancouver, BC
We use our routine antigen retrieval technique on our FISH for Her2.
Specifically we use 25 minutes in Dako's Target Retrieval in our bench top
Autoclave. So far our results are good. Tom.
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