Re: Pretreatment for in situ hybridization
We are using heat for the EBER1 probe. We pretreat in 10mM citrate, pH
6.0 in a microwave pressure cooker for 15 minutes at pressure. We did
comparisons between the above method and Proteinase K and Pepsin. The HIER
was far superior. I don't know about B-5, or the other probes, we haven't
Best of luck,
PhenoPath Laboratories--Seattle, WA
on 6/6/02 8:35 PM, C Sawrenko at firstname.lastname@example.org wrote:
> Hello Histonetters,
> Does anyone out there have any experience using heat (by steam, microwave or
> pressure cooker) instead of enzyme (Proteinase K) for pretreatment prior to
> in situ hybridization? We are attempting ISH for kappa, lambda and EBER.
> Also has anyone had any success on B5 fixed tissue for the above?
> Thanks in advance for your help!
> Christina Sawrenko
> BC Cancer Agency
> Vancouver, BC
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