Re: Neuromuscular junctions
There are many modifications available for the original (Karnovsky & Roots)
enzyme histochemistry techniques. These enzyme histochemistry techniques can
be found in the Histochemistry "bibles" of both Pearse and Lillie. Some
histology technique books also contain these methods. The standard protocol
for fixation calls for cold formalin (various formulas) followed by
cryosectioning. You can not do paraffin for the enzyme methods.
I am not that familiar with the antibodies that you have mentioned. The
manufacturer's recommended method does not work? Perhaps the muscle sections
are not in the proper plane to see neuromuscular junction?
University of Washington
----- Original Message -----
Sent: Wednesday, June 12, 2002 9:52 AM
Subject: Neuromuscular junctions
> Hi everyone
> Does anyone have a working protocol(IHC or special stain) to detect
neuromuscular junctions in formalin-fixed rat muscles?! I have tried
antibodies to nicotinic receptors alpha-4, beta-1, beta-2, as well as
acetylcholinesterase, all to no avail. I am contemplating releasing my
newest scientific finding that rats lack neuromuscular junctions
> Any help would be greatly appreciated!
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