Re: NBF penetration rate

From:Patrick Foley


Helander (1999) performed a kinetic study of 4% buffered formalin
fixation in rat brains and kidneys at 24 C.  The author found that: 

- The rate of fixation was 80 microns per hour.
- Half-maximal binding was reached in 3 hours for kidney and 5 hours
for brain.
- Maximal binding was reached after about 50 hours
- Maximal formaldehyde binding required about 10 hours per mm thickness
of tissue.

Therefore, the rate of penetration and the rate of maximal fixation are
not equal.  Fixation occurs at a slower rate than penetration. 
Helander's paper may be of interest if you are also concerned about the
rate of fixation.

Helander, Kerstin G.  1999.  Formaldehyde binding in brain and kidney:
A kinetic study of fixation.  The Journal of Histotechnology 22(4):

Patrick Foley
Scott & White
Temple, TX

>>> "Connolly, Brett M"  06/05/02 10:50AM
Can someone give me the tissue penetration rate of  10% NBF in mm/hr.
room temp. 

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Neuroscience
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075

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