Dear Agustin

PCNA has been used in several studies.  I'm beginning a mitotic count
myself and have just begun using anti-PCNA.  I can tell you so far that
you need to run your dilution series out until you see nothing.  The
protein is apparently always present in the nucleus so you'll see some
signal.  In S phase, PCNA is upregulated so those nuclei will be heavily
labeled when compared to the lighter labeling in other nuclei.  Or at
least that's what I'm seeing at this early stage.

If anyone out there knows that I'm wandering up a cul du sac, let me know
won't you?

Yours counting from the top,

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Mon, 3 Jun 2002, [UNKNOWN] Agustín Venzano wrote:

> Dear netters: I'm planning a sampling of ruminal papillae (i.e. special
> structures endowed with epithelium and lamina propria located in the largest
> forestomach of ruminants) in young cattle. The project is aimed at defining
> the growth rate of these structures specialised in nutrients absorption, so
> it is necessary to estimate the mitotic index. My questions are:
>
> 1.What staining would you prefer for seeing DNA and mitosis?
> 2. Do you consider c-kit to be an adequate marker of the mitotic rate
> through IHC in paraffin blocks?
>
> Thank you in advance
>
> Sincerely yours
>
> Agustin Jose Venzano Halliburton
> DVM-Pathology Group
> INTA, Argentina
>
>
>