Re: Hair specimen
that will be tough.
i would try slides precoated with 5% Elmer's type glue. i mix
the glue with dh20 and dip the slides in it, stand them up to airdry, then
i use these slides to pick up the sections, dry and heat slides as usual.
for IHC you have to be aware that some antibodies will react with the glue
as it is a biologically active, collagen antibodies are especially reactive
to the glue.
another suggestion would be the Intermedics tape transfer system, where
you pick up the section attached to tape, roll the tape/section onto a
polymer coated slide, expose the slide to UV light then remove the tape
and the section is left on the slide.
"Polkovitch, Debbie [PRDUS]" wrote:
I was wondering if anyone has helpful hints on keeping
Hair (human) on a slide. We have used positively charged slides,
have run the slides through xylene to diH2O to remove the paraffin, and
have tried microwave heat, water bath heat (95 degrees) and no heat at
all for antigen retrieval. We would like to run IHC stains, but can't
seem to keep the specimen on the slide. Any suggestions?
Thanks to all in advance!
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