Re: Bouins Fixation

From:Geoff McAuliffe

Hi Louise:

    I seem to remember that the various components of Bouin's penetrate at
their own rates but 5 days would seem to be enough to get the center throughly
(if not well) fixed. I would suspect inadquate infiltration. Perhaps embedding
a similarly sized and fixed piece in wax would tell the story?

louise renton wrote:

> Dear All,
> I need some advice please.
> We are currently using Bouin's fixative to fix rat tissue samples of muscle
> and bone. The samples are approximately 1cm x 0.5cm and were fixed for 5
> days prior to infiltration with Historesin (GMA). When cut, however, the
> centres were soggy and uncutable. Is this a problem with fixation or with
> the infiltration time being insufficient or both?. I seem to remember that
> way back when when I was learning about such, that Bouins has poor
> penetration, but excellent cytological preservation. What are the comments
> and thoughts of the forum?
> _________________________________________________________________
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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