Re: Alzeheimers stain

From:Melissa Jensen
Re: Alzeheimers stain
Thanks much Joan! I am wondering now if there is a different silver method to use.The reagents for Bielsowskys are numerous.Not sure I want to spend that kind of money for a stain we may only use occasionally.Any recommendations you have would be appreciated!
----- Original Message -----
From: Joan Yonchek
To: 'Melissa Jensen'
Sent: Thursday, June 13, 2002 4:38 PM
Subject: RE: Alzeheimers stain

Melissa,
 
Just sent the fax.  If you try the procedure let me know how it turns out.
 
Good luck,
 
Joan
Regeneration Technologies, Inc
Alachua, FL  32615
-----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: Wednesday, June 12, 2002 6:33 PM
To: Joan Yonchek
Subject: Re: Alzeheimers stain

That would be great! Fax is 319-366-6976
----- Original Message -----
From: Joan Yonchek
To: 'Melissa Jensen'
Sent: Tuesday, June 11, 2002 7:59 AM
Subject: RE: Alzeheimers stain

Melissa,
 
Microwave Cookbook for Microscopists
Art and Science of Visualization
By  Kok and Boon
 
Chapter 16, page 250
Procedure 16.15  Microwave-Stimulated King Silver Staining for paraffin-embedded tissues.
Results:  Neurofibullary tangles and senile plaques are black.
The procedure states:  The core of the plaque is difficult to stain.  The Alzheimer's fibrils will not always be colored.  Lloyd et al. published in 1985 a microwave method. We changed their method slightly.
                        
I have never tried this procedure but would be happy to fax you a copy.
 
Joan
Regeneration Technologies, Inc
Alachua, FL  32615

 

At 6:21 PM -0500 9/6/02, Melissa Jensen wrote:
Looking for a microwave stain to demonstrate plaque in the brain.....Can ya help me out?

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