RE: nuking slides

From:

"Smith, Allen"

The microwave doesn't heat the paraffin; it heats the water under the paraffin. Since the water under the center boils away last, the center of the section heats longest and gets hottest.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695

-----Original Message-----
From: virginia brown [mailto:histodiva67@msn.com]
Sent: Thursday, June 13, 2002 7:05 PM
To: histonet@pathology.swmed.edu
Subject: nuking slides

is anyone micro waving their slides prior to staining in lieu of a "regular" oven? if so, what setting, wattage, length of time and placement on the revolving plate etc.? the lab i work for is "suddenly" having uneven staining , appearing as though the paraffin is not heating evenly. hence the h & e can't stain because the paraffin is inhibiting. thanks in advance.. lynn brown

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