Hi Ronda-

What anti-luciferase antibody do you use on paraffin embedded, PFA fixed
tissues?  Are there any tricks, like antigen retrieval, length of fixation
required, for good staining?

Thanks,
Ralph

Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945
Fax: 415-476-1128


-----Original Message-----
From: Ronda Schreiber [mailto:rondas@selectivegenetics.com]
Sent: Thursday, June 06, 2002 10:14 AM
To: HistoNet Server
Subject: 


Hello,

I am deparately searching for a validated method for luciferase
immunostaining of pfa fixed, formic acid demineralized, paraffin embedded
tissue. Our laboratory has successfully stained PFA fixed, paraffin embedded
tissues, but never demineralized tissues. Would like to know if there are
any special antigen retrieval methods out there, etc...
Thanks much,


Ronda Schreiber, PhD
Associate Director, Orthopedic Tissue Repair

Selective Genetics
11035 Roselle St.
San Diego, CA 92121
rondas@selectivegenetics.com
(858) 625-0100 phone
(858) 625-0050 fax