We use Dysferlin from Novacastra at 1:5 for 75 mins incubation and visualise
with Dako LSAB2 kit on frozen muscle sections. No fixation or pretreatments
are used. Our medics seem happy with the results.

Bob Quilty
Dept of Neuropathology
Frenchay Hospital
Bristol UK

-----Original Message-----
From: Barbara Terrett [mailto:histobabs@hotmail.com]
Sent: 14 June 2002 16:25
To: histonet@pathology.swmed.edu
Subject: dysferlin(Hamlet)



Hi all,
Is anyone using Dysferlin (Hamlet and Hamlet-2)on frozen muscle sections? We

are trying some out, and not quite happy with our results yet. What are 
others doing as far as fixation, pretreatment etc.?
b


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