We use Dysferlin from Novacastra at 1:5 for 75 mins incubation and visualise
with Dako LSAB2 kit on frozen muscle sections. No fixation or pretreatments
are used. Our medics seem happy with the results.
Dept of Neuropathology
From: Barbara Terrett [mailto:email@example.com]
Sent: 14 June 2002 16:25
Is anyone using Dysferlin (Hamlet and Hamlet-2)on frozen muscle sections? We
are trying some out, and not quite happy with our results yet. What are
others doing as far as fixation, pretreatment etc.?
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