RE: buffer preservative ?

From:Tony Henwood

RE: buffer preservative ?

Sodium Azide is allright for the reactions but it does block peroxidase. So better to not add it to buffers or peroxidase conjugated reagents and especially not to the DAB-H2O2 substrate.

Nina leek (Histonet Wednesday, 28 November) preferred to use Kathon, which she obtained from Rohm & Haas.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

 

-----Original Message-----
From: rueggp [mailto:rueggp@earthlink.net]
Sent: Thursday, 6 June 2002 13:22
To: Laura Bliven
Cc: histonet@pathology.swmed.edu
Subject: Re: buffer preservative ?


years ago we used to add a tiny amount of sodium azide to our diluent, but you have to be careful as it can inhibit ihc reactions.  i will try and find the concentration used.

patsy ruegg

Laura Bliven wrote:

> I would like to add a preservative to our Tris Buffered Saline Buffer, pH 7.6, but am unsure as to what chemical and how much.  The staining will be immunohistochemistries and I know some chemicals are incompatible.

> Thanks,
> Laura



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