Morning Andrea,

	It has been suggested that my response to message below erroneously
pointed finger at YOU rather than "research tech".  If that was YOUR
interpretation, I apologize for misdirection of 'finger point' and for
misallocation of fault.  Further, since "fault" placement was not intention
but, rather, only late-week fun, if FUN was not received by you, or
"research tech", I submit further humble apology.

	In addition, I hope that the excess copper sulfate has diffused from
the tissue by now.

Regards,

Fred

> ----------
> From: 	Andrea Grantham
> Sent: 	Thursday, May 30, 2002 11:17 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	WEIRD FIXATIVE
> 
> One of the research techs here brought some tumor tissue to the lab fixed 
> in Copper Formalin.
> In one of his replys to my quizzing him about the fixative he said that he
> 
> wanted to make up zinc formalin but didn't have any zinc and "copper is 
> right next to zinc" (on the chart, I presume) so he just thought he'd give
> 
> it a try.
> What might we expect in terms of staining?
> When we bisected the tumors to process it was interesting to see that
> there 
> was a turquoise color in the center of the tissue. None of the other
> tumors 
> fixed in other fixatives  (10% NBF, Carnoys) had this color.
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
> 
> 
>