RE: Pretreatment for in situ hybridization

From:"Sebree Linda A."


Our EBER, kappa & lambda on B5-fixed bone marrow biopsies are beautiful
using Ventana instruments.  Let me know if you need/want details.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
From: C Sawrenko []
Sent: Thursday, June 06, 2002 10:36 PM
To: HistoNet Server
Subject: Pretreatment for in situ hybridization

Hello Histonetters,
Does anyone out there have any experience using heat (by steam, microwave or
pressure cooker) instead of enzyme (Proteinase K) for pretreatment prior to
in situ hybridization?  We are attempting ISH for kappa, lambda and EBER.
Also has anyone had any success on B5 fixed tissue for the above?
Thanks in advance for your help!

Christina Sawrenko
BC Cancer Agency
Vancouver, BC

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