Pretreatment for in situ hybridization

From:C Sawrenko

Hello Histonetters,
Does anyone out there have any experience using heat (by steam, microwave or
pressure cooker) instead of enzyme (Proteinase K) for pretreatment prior to
in situ hybridization?  We are attempting ISH for kappa, lambda and EBER.
Also has anyone had any success on B5 fixed tissue for the above?
Thanks in advance for your help!

Christina Sawrenko
BC Cancer Agency
Vancouver, BC

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