Hair specimen

From:"Polkovitch, Debbie [PRDUS]"

Hair specimen


I was wondering if anyone has helpful hints on keeping Hair (human) on a slide.  We have used positively charged slides, have run the slides through xylene to diH2O to remove the paraffin, and have tried microwave heat, water bath heat (95 degrees) and no heat at all for antigen retrieval.  We would like to run IHC stains, but can't seem to keep the specimen on the slide.  Any suggestions?  

Thanks to all in advance!

Deborah Polkovitch, MT (ASCP)
Research Associate; Drug Discovery; Rm. DSE-435
Johnson and Johnson PRD
Welsh and McKean Rds., P.O. Box 776
Spring House, PA 19477-0776
(215) 628-5392

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